Espinosa, Javier and Brunner, Thomas and Fiedler, Nicole and Forchhammer, Karl and Muro-Pastor, Alicia M. and Maldener, Iris (2010) DevT (Alr4674), resembling a Ser/Thr protein phosphatase, is essential for heterocyst function in the cyanobacterium Anabaena sp PCC 7120. MICROBIOLOGY-SGM, 156. pp. 3544-3555. ISSN 1350-0872, 1465-2080
Full text not available from this repository. (Request a copy)Abstract
Heterocyst-forming cyanobacteria are able to perform oxygenic photosynthesis and nitrogen fixation simultaneously in the same filament, by restricting the highly O-2-sensitive nitrogenase to specialized cells, the heterocysts. A remarkable change in morphology and metabolism accompanies the differentiation of heterocysts, which only occurs when no source of combined nitrogen is available. In this study, we characterized DevT (Alr4674), a putative protein phosphatase from Anabaena PCC 7120. Mutants defective in devT are able to form morphologically mature heterocysts, which however cannot fix N-2, and the mutant cannot survive without a source of combined nitrogen. DevT shows homology to phosphatases of the PPP family and displays a Mn2+-dependent phosphatase activity that can be inhibited by phosphatase inhibitors and oxidizing conditions. DevT is constitutively expressed in both vegetative cells and heterocysts, and is not regulated by NtcA. The heterocyst regulator HetR may exert a certain inhibition on the expression of devT. Under diazotrophic growth conditions, DevT protein accumulates specifically in mature heterocysts. Therefore DevT plays a still unknown role in a late essential step of heterocyst differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SP-STRAIN PCC-7120; SERINE THREONINE PHOSPHATASES; BLUE-GREEN-ALGAE; SERINE/THREONINE PHOSPHATASES; FILAMENTOUS CYANOBACTERIA; ESCHERICHIA-COLI; GENE; DIFFERENTIATION; HETR; NTCA; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 09:37 |
| Last Modified: | 06 Jul 2020 09:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23810 |
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