Armache, Jean-Paul and Jarasch, Alexander and Anger, Andreas M. and Villa, Elizabeth and Becker, Thomas and Bhushan, Shashi and Jossinet, Fabrice and Habeck, Michael and Dindar, Guelcin and Franckenberg, Sibylle and Marquez, Viter and Mielke, Thorsten and Thomm, Michael and Berninghausen, Otto and Beatrix, Birgitta and Soeding, Johannes and Westhof, Eric and Wilson, Daniel N. and Beckmann, Roland (2010) Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-angstrom resolution. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107 (46). pp. 19748-19753. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution. This map, together with a 6.1-angstrom map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model similar to 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 5.5 ANGSTROM RESOLUTION; ELECTRON-MICROSCOPY; MOLECULAR-DYNAMICS; MESSENGER-RNA; RAT-LIVER; SECONDARY STRUCTURE; EXPANSION SEGMENTS; CROSS-LINKING; 70S RIBOSOME; EXIT TUNNEL; modeling; molecular dynamics; flexible fitting |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 11:11 |
| Last Modified: | 06 Jul 2020 11:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23876 |
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