Armache, Jean-Paul and Jarasch, Alexander and Anger, Andreas M. and Villa, Elizabeth and Becker, Thomas and Bhushan, Shashi and Jossinet, Fabrice and Habeck, Michael and Dindar, Guelcin and Franckenberg, Sibylle and Marquez, Viter and Mielke, Thorsten and Thomm, Michael and Berninghausen, Otto and Beatrix, Birgitta and Soeding, Johannes and Westhof, Eric and Wilson, Daniel N. and Beckmann, Roland (2010) Localization of eukaryote-specific ribosomal proteins in a 5.5-angstrom cryo-EM map of the 80S eukaryotic ribosome. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107 (46). pp. 19754-19759. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution, together with a 6.1-angstrom map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIPLE SEQUENCE ALIGNMENT; 5.5 ANGSTROM RESOLUTION; INITIATION-FACTOR EIF-3; VIRUS IRES RNA; MESSENGER-RNA; CROSS-LINKING; SACCHAROMYCES-CEREVISIAE; TRANSLATION INITIATION; STRUCTURE PREDICTION; SPATIAL ARRANGEMENT; homology modeling; RNA; translation; flexible fitting; molecular dynamics |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 11:15 |
| Last Modified: | 06 Jul 2020 11:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23877 |
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