Scherer, Max and Schmitz, Gerd and Liebisch, Gerhard (2010) Simultaneous Quantification of Cardiolipin, Bis(monoacylglycero)phosphate and their Precursors by Hydrophilic Interaction LC-MS/MS Including Correction of Isotopic Overlap. ANALYTICAL CHEMISTRY, 82 (21). pp. 8794-8799. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Cardiolipin (CL) and bis(monoacylglycero)phosphate (BMP) are unique lipid structures with important biological roles for mitochondrial integrity and endolysosomal degradation, respectively. They are synthesized from common precursors, phosphatidylglycerol (PG) and phosphatidic acid (PA). Here we present a rapid method for the simultaneous quantification of BMP, CL, PG, and PA using hydrophilic interaction chromatography coupled with electrospray ionization tandem mass spectrometry (HILIC-MS/MS). HILIC provides coelution of lipid species and their internal standards required for accurate quantification. Coelution leads to isotope overlap of lipid species which was successfully corrected. This assay was validated in mouse heart tissue and primary human skin fibroblasts. It shows reproducibility and limits of detection sufficient for biomarker studies contributing to basic research on BMP and CL metabolism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHROMATOGRAPHY-MASS SPECTROMETRY; ELECTROSPRAY-IONIZATION; QUANTITATIVE-ANALYSIS; SHOTGUN LIPIDOMICS; LYSOPHOSPHATIDIC ACID; MULTIPLE PRECURSOR; BIOLOGICAL SAMPLES; MEMBRANE-LIPIDS; BARTH-SYNDROME; IDENTIFICATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jul 2020 13:14 |
| Last Modified: | 06 Jul 2020 13:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23910 |
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