Schmid, R. and Schiffner, S. and Opolka, A. and Graessel, S. and Schubert, T. and Moser, M. and Bosserhoff, Anja-Katrin (2010) Enhanced cartilage regeneration in MIA/CD-RAP deficient mice. CELL DEATH & DISEASE, 1: e97. ISSN 2041-4889,
Full text not available from this repository. (Request a copy)Abstract
Melanoma inhibitory activity/cartilage-derived retinoic acid-sensitive protein (MIA/CD-RAP) is a small soluble protein secreted from chondrocytes. It was identified as the prototype of a family of extracellular proteins adopting an SH3 domain-like fold. In order to study the consequences of MIA/CD-RAP deficiency in detail we used mice with a targeted gene disruption of MIA/CD-RAP (MIA-/-) and analyzed cartilage organisation and differentiation in in vivo and in vitro models. Cartilage formation and regeneration was determined in models for osteoarthritis and fracture healing in vivo, in addition to in vitro studies using mesenchymal stem cells of MIA -/- mice. Interestingly, our data suggest enhanced chondrocytic regeneration in the MIA -/- mice, modulated by enhanced proliferation and delayed differentiation. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa (p54(nrb)), a recently described modulator of Sox9 activity. In this study, we present p54(nrb) as a mediator of MIA/CD-RAP to promote chondrogenesis. Taken together, our data indicate that MIA/CD-RAP is required for differentiation in cartilage potentially by regulating signaling processes during differentiation. Cell Death and Disease (2010) 1, e97; doi: 10.1038/cddis.2010.78; pubished online 11 November 2010
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MELANOMA INHIBITORY-ACTIVITY; SENSITIVE PROTEIN GENE; CHONDROCYTE DIFFERENTIATION; CELL-LINES; EXPRESSION; CHONDROGENESIS; PROLIFERATION; TRANSCRIPTION; PROMOTER; CLONING; cartilage regeneration; MIA/CD-RAP; differentiation; cell signalling; proliferation; MIA-knockout mouse |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2020 06:31 |
| Last Modified: | 07 Jul 2020 06:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23921 |
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