Schmidt, Jennifer and Friebel, Kristin and Schoenherr, Roland and Coppolino, Marc G. and Bosserhoff, Anja-Katrin (2010) Migration-associated secretion of melanoma inhibitory activity at the cell rear is supported by KCa3.1 potassium channels. CELL RESEARCH, 20 (11). pp. 1224-1238. ISSN 1001-0602, 1748-7838
Full text not available from this repository. (Request a copy)Abstract
Malignant melanoma, characterized by invasive local growth and early formation of metastases, is the most aggressive type of skin cancer. Melanoma inhibitory activity (MIA), secreted by malignant melanoma cells, interacts with the cell adhesion receptors, integrins alpha(4)beta(1) and alpha(5)beta(1), facilitating cell detachment and promoting formation of metastases. In the present study, we demonstrate that MIA secretion is confined to the rear end of migrating cells, while in non-migrating cells MIA accumulates in the actin cortex. MIA protein takes a conventional secretory pathway including coat protein complex I (COPI)- and coat protein complex II (COPII)-dependent protein transport to the cell periphery, where its final release depends on intracellular Ca2+ ions. Interestingly, the Ca2+-activated K+-channel, subfamily N, member 4 (KCa3.1), known to be active at the rear end of migrating cells, was found to support MIA secretion. Secretion was diminished by the specific KCa3.1 channel inhibitor TRAM-34 and by expression of dominant-negative mutants of the channel. In summary, we have elucidated the migration-associated transport of MIA protein to the cell rear and also disclosed a new mechanism by which KCa3.1 potassium channels promote cell migration.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CA2+-ACTIVATED K+ CHANNELS; FIBROBLAST GROWTH FACTOR-2; INTRA-GOLGI TRANSPORT; CANINE KIDNEY-CELLS; MALIGNANT-MELANOMA; ENDOPLASMIC-RETICULUM; MATRIX-METALLOPROTEINASE; CYCLE PROGRESSION; EPITHELIAL-CELLS; ION CHANNELS; MIA protein; KCa3.1 potassium channel; migration; directed transport; regulated secretion |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2020 06:32 |
| Last Modified: | 07 Jul 2020 06:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23922 |
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