Garrett, Roger A. and Prangishvili, David and Shah, Shiraz A. and Reuter, Monika and Stetter, Karl O. and Peng, Xu (2010) Metagenomic analyses of novel viruses and plasmids from a cultured environmental sample of hyperthermophilic neutrophiles. ENVIRONMENTAL MICROBIOLOGY, 12 (11). pp. 2918-2930. ISSN 1462-2912,
Full text not available from this repository. (Request a copy)Abstract
P>Two novel viral genomes and four plasmids were assembled from an environmental sample collected from a hot spring at Yellowstone National Park, USA, and maintained anaerobically in a bioreactor at 85 degrees C and pH 6. The double-stranded DNA viral genomes are linear (22.7 kb) and circular (17.7 kb), and derive apparently from archaeal viruses HAV1 and HAV2. Genomic DNA was obtained from samples enriched in filamentous and tadpole-shaped virus-like particles respectively. They yielded few significant matches in public sequence databases reinforcing, further, the wide diversity of archaeal viruses. Several variants of HAV1 exhibit major genomic alterations, presumed to arise from viral adaptation to different hosts. They include insertions up to 350 bp, deletions up to 1.5 kb, and genes with extensively altered sequences. Some result from recombination events occurring at low complexity direct repeats distributed along the genome. In addition, a 33.8 kb archaeal plasmid pHA1 was characterized, encoding a possible conjugative apparatus, as well as three cryptic plasmids of thermophilic bacterial origin, pHB1 of 2.1 kb and two closely related variants pHB2a and pHB2b, of 5.2 and 4.8 kb respectively. Strategies are considered for assembling genomes of smaller genetic elements from complex environmental samples, and for establishing possible host identities on the basis of sequence similarity to host CRISPR immune systems.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THERMOPROTEUS-TENAX; ARCHAEAL VIRUSES; GEOTHERMAL ENVIRONMENTS; MECHANISM; FAMILY; SULFOLOBUS; GENOME; RESISTANCE; PARTICLES; DIVERSITY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2020 06:42 |
| Last Modified: | 07 Jul 2020 06:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23926 |
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