Peter, Emanuel and Dick, Bernhard and Baeurle, Stephan A. (2010) Mechanism of signal transduction of the LOV2-J alpha photosensor from Avena sativa. NATURE COMMUNICATIONS, 1: 122. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Fusion proteins containing blue-light-activable protein domains possess great potential as molecular switches in cell signalling. This has recently been impressively demonstrated by connecting the light oxygen voltage LOV2-J alpha-protein domain of A. sativa (AsLOV2-J alpha) with the Rac1-GTPase, responsible for regulating the morphology and motility of metazoan cells. However, a target-oriented development of fusion proteins in conjunction with this photosensor is still very challenging, because a detailed understanding of its signal transduction pathway on a molecular level is still lacking. Here, we show through molecular dynamics simulation that, after formation of the cysteinyl-flavin mononucleotide (FMN) adduct, the signalling pathway begins with a rotational reorientation of the residue glutamine 1029 adjacent to the FMN chromophore, transmitting stress through the I beta strand towards the LOV2-J alpha interface. This then results in the breakage of two H-bonds, namely, glutamic acid 1034-Gln995 and aspartic acid (Asp) 1056-Gln1013, at opposite sides of the interface between the J alpha helix and the LOV2 domain, ultimately leading to a disruption of J alpha helix from the LOV2 core.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | J-ALPHA HELIX; LOV2 DOMAIN; STRUCTURAL-CHANGES; PHOTOTROPIN; SIMULATION; TIME; CHROMOPHORE; PHOTOCYCLE; PHOT-LOV1; FTIR; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Molecular Spectroscopy and Photochemistry) > Prof. Dr. Bernhard Dick Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Molecular Spectroscopy and Photochemistry) > PD Dr. Stephan Baeurle |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jul 2020 12:56 |
| Last Modified: | 07 Jul 2020 12:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23971 |
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