Steiner, Mark-Steven and Meier, Robert J. and Duerkop, Axel and Wolfbeis, Otto S. (2010) Chromogenic Sensing of Biogenic Amines Using a Chameleon Probe and the Red-Green-Blue Readout of Digital Camera Images. ANALYTICAL CHEMISTRY, 82 (20). pp. 8402-8405. ISSN 0003-2700, 1520-6882
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We report on sensing spots containing an amine reactive chromogenic probe and a green fluorescent (amine insensitive) reference dye incorporated in a hydrogel matrix on a solid support. Such spots enable rapid and direct determination of primary amines and, especially, biogenic amines (BA). A distinct color change from blue to red occurs on dipping the test spots into a pH 9.0 sample containing primary amines. BAs can be determined in the concentration range from 0.01 to 10 mM within 15 min, enabling rapid, qualitative, and semiquantitative evaluation. In the "photographic" approach, the typically 4-7.5-fold increase in fluorescence intensity of the probe at 620 nm along with the constant green fluorescence at 515 nm of a reference dye are used for quantitation of BAs. The sensing spots are photoexcited with high-power 505 nm light-emitting diodes (LEDs) in a black box. A digital picture is acquired with a commercially available digital camera, and the color information is extracted via red-green-blue (RGB) readout. The ratio of the intensities of the red (signal) channel and the green (reference) channel yields pseudocolor pictures and calibration plots.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLORIMETRIC SENSOR; DERIVATIZATION; HISTAMINE; FISH; DISCRIMINATION; CHROMATOGRAPHY; POLYAMINES; PROTEINS; LABELS; ARRAY; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jul 2020 06:28 |
| Last Modified: | 08 Jul 2020 06:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24007 |
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