Zaky, A. and Elbakry, A. and Ehmer, A. and Breunig, M. and Goepferich, Achim (2010) The mechanism of protein release from triglyceride microspheres. JOURNAL OF CONTROLLED RELEASE, 147 (2). pp. 202-210. ISSN 0168-3659, 1873-4995
Full text not available from this repository. (Request a copy)Abstract
The purpose of this study was to reveal factors that have an impact on the protein release kinetics from triglyceride microspheres prepared by spray congealing. We investigated the effect of protein particle size, morphology and distribution on protein release from microspheres by confocal laser scanning microscopy (CLSM). The microspheres were loaded with three types of model particles made of FITC-labeled bovine serum albumin: freeze dried protein, spherical particles obtained by precipitation in the presence of PEG and micronized material. Investigation by light microscopy and laser light diffraction revealed that the freeze dried material consisted mainly of app. 29 mu m elongated shaped particles. The precipitated BSA consisted mainly of 9.0 mu m diameter spherically shaped particles while the micronized protein prepared by jet milling consisted of 4.9 mu m sized rounded particles of high uniformity. Microspheres were embedded into a cold-curing resin and cut with a microtome. Subsequent investigation by CLSM revealed major differences of distribution of the polydisperse protein particles inside the microsphere sections depending on the type of BSA that was used. Particles of micronized and precipitated protein were distributed almost throughout the microsphere cross section. The protein distribution had a marked impact on the release kinetics in phosphate buffer. Large protein particles led to a considerably faster release than small ones. By staining the release medium we demonstrated that in all three cases there was a strong correlation between protein release and buffer intrusion. (C) 2010 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SCANNING ELECTRON-MICROSCOPY; SPRAY-CONGEALING PROCESS; DRUG-RELEASE; LIPID MICROPARTICLES; VERAPAMIL HYDROCHLORIDE; SUSTAINED-RELEASE; IN-VITRO; MATRICES; IMPLANTS; BEHAVIOR; Lipid microspheres; Confocal microscopy; Particle distribution; Protein release; Release mechanism |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jul 2020 06:55 |
| Last Modified: | 08 Jul 2020 06:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24010 |
Actions (login required)
 |
View Item |
