Freischmidt, Axel and Meysing, Maren and Liss, Michael and Wagner, Ralf and Kalbitzer, Hans Robert and Horn, Gudrun (2010) Limiting factors of the translation machinery. JOURNAL OF BIOTECHNOLOGY, 150 (1). pp. 44-50. ISSN 0168-1656, 1873-4863
Full text not available from this repository. (Request a copy)Abstract
Cell-free production of proteins is a rapid developing technology for many applications in proteomic sciences. Although great progress was made in the last years, in vitro protein synthesis is still less efficient than in vivo protein synthesis. For Escherichia colt based systems, all factors needed for cell-free protein synthesis are established, as shown in the reconstituted PURE system. Here we report the influence of additional translation factors, aminoacyl-tRNA synthetases and translational active ribosomes on cell-free protein synthesis of E. coli S30 extracts, indicating that none of these factors is a limiting factor for the protein synthesis. Furthermore, we show that varying the ratio of ribosomes including associated factors to other factors present in the extracts could not increase the yield of protein synthesized. In summary our results provide strong evidence for an optimal reconstitution of the translation machinery in E. coli S30 extracts. (C) 2010 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FREE PROTEIN-SYNTHESIS; CELL-FREE PRODUCTION; SYNTHESIS SYSTEM; ESCHERICHIA-COLI; HIGHLY EFFICIENT; HIGH-THROUGHPUT; POLYPEPTIDES; EXPRESSION; MEMBRANE; SCALE; Cell-free protein synthesis; Translation factors; Escherichia coli; S30 extract |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jul 2020 10:13 |
| Last Modified: | 09 Jul 2020 10:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24083 |
Actions (login required)
 |
View Item |
