Sommer, Ulf and Petersen, Jorgen and Pfeiffer, Michael and Schrotz-King, Petra and Morsczeck, Christian (2010) Comparison of surface proteomes of enterotoxigenic (ETEC) and commensal Escherichia coli strains. JOURNAL OF MICROBIOLOGICAL METHODS, 83 (1). pp. 13-19. ISSN 0167-7012, 1872-8359
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Pathogenesis of enterotoxigenic Escherichia coli (ETEC) infections involves colonization of the small intestine mediated by cell-surface fimbriae (CS) or colonization fimbriae antigens (CFA). However, protection against reinfection of ETEC is also conferred by somatic antigens rather than by virulence factors. To discover ETEC specific somatic antigens, the surface proteome of the ETEC H10406 strain was compared with that of nonpathogenic E. coli K12 strains. In this study, we were using stable isotope labelling with amino acids in cell culture (SILAC) technology for the labelling and relative quantification of surface proteins in order to identify polypeptides that are specifically present on ETEC strains. Outer membrane proteins were isolated, separated by gel electrophoresis, and identified by mass spectrometry. Twenty-three differentially expressed cell-surface polypeptides of ETEC were identified and evaluated by bioinformatics for protein vaccine candidates. The combination of being surface-exposed and present differentially makes these polypeptides highly suitable as targets for antibodies and thus for use in passive or active immunisation/vaccination. (C) 2010 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CAMPYLOBACTER-JEJUNI; TRAVELERS DIARRHEA; FLAGELLIN; VACCINE; COLONIZATION; PROTEINS; CELLS; SILAC; Escherichia coli; Surface proteins; Vaccine; Proteomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jul 2020 10:46 |
| Last Modified: | 09 Jul 2020 10:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24091 |
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