Zhu, Jia Yun and Strehle, Martin and Frohn, Anne and Kremmer, Elisabeth and Hoefig, Kai P. and Meister, Gunter and Adler, Heiko (2010) Identification and Analysis of Expression of Novel MicroRNAs of Murine Gammaherpesvirus 68. JOURNAL OF VIROLOGY, 84 (19). pp. 10266-10275. ISSN 0022-538X,
Full text not available from this repository. (Request a copy)Abstract
Murine gammaherpesvirus 68 (MHV-68) is closely related to Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) and provides a small-animal model with which to study the pathogenesis of gammaherpesvirus (gamma HV) infections. To completely explore the potential of the MHV-68 system for the investigation of gamma HV microRNAs (miRNAs), it would be desirable to know the number and expression patterns of all miRNAs encoded by MHV-68. By deep sequencing of small RNAs, we systematically investigated the expression profiles of MHV-68 miRNAs in both lytically and persistently infected cells. In addition to the nine known MHV-68 miRNAs, we identified six novel MHV-68 miRNA genes and analyzed the expression levels of all MHV-68 miRNAs. Furthermore, we also characterized the cellular miRNA expression signatures in MHV-68-infected versus noninfected NIH 3T3 fibroblasts and in 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated versus nontreated S11 cells. We found that mmu-mir-15b and mmu-mir-16 are highly upregulated upon MHV-68 infection of NIH 3T3 cells, indicating a potential role for cellular miRNAs during MHV-68 infection. Our data will aid in the full exploration of the functions of gamma HV miRNAs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SARCOMA-ASSOCIATED HERPESVIRUS; VIRUS-ENCODED MICRORNAS; GAMMA-HERPESVIRUS; VIRAL MICRORNAS; MOUSE MODEL; INFECTIOUS-MONONUCLEOSIS; RT-PCR; RNA; TARGETS; PATHOGENESIS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Jul 2020 12:25 |
| Last Modified: | 09 Jul 2020 12:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24098 |
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