Lamina, Claudia and Kuechenhoff, Helmut and Chang-Claude, Jenny and Paulweber, Bernhard and Wichmann, H. -Erich and Illig, Thomas and Hoehe, Margret R. and Kronenberg, Florian and Heid, Iris M. (2010) Haplotype Misclassification Resulting from Statistical Reconstruction and Genotype Error, and Its Impact on Association Estimates. ANNALS OF HUMAN GENETICS, 74. pp. 452-462. ISSN 0003-4800, 1469-1809
Full text not available from this repository. (Request a copy)Abstract
P>Haplotypes are an important concept for genetic association studies, but involve uncertainty due to statistical reconstruction from single nucleotide polymorphism (SNP) genotypes and genotype error. We developed a re-sampling approach to quantify haplotype misclassification probabilities and implemented the MC-SIMEX approach to tackle this as a 3 x 3 misclassification problem. Using a previously published approach as a benchmark for comparison, we evaluated the performance of our approach by simulations and exemplified it on real data from 15 SNPs of the APM1 gene. Misclassification due to reconstruction error was small for most, but notable for some, especially rarer haplotypes. Genotype error added misclassification to all haplotypes resulting in a non-negligible drop in sensitivity. In our real data example, the bias of association estimates due to reconstruction error alone reached -48.2% for a 1% genotype error, indicating that haplotype misclassification should not be ignored if high genotype error can be expected. Our 3 x 3 misclassification view of haplotype error adds a novel perspective to currently used methods based on genotype intensities and expected number of haplotype copies. Our findings give a sense of the impact of haplotype error under realistic scenarios and underscore the importance of high-quality genotyping, in which case the bias in haplotype association estimates is negligible.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LINKAGE PHASE; UNCERTAINTY; TRAITS; TESTS; GENE; SUSCEPTIBILITY; FREQUENCIES; POPULATION; SAMPLE; Haplotypes; measurement error; genotyping error; association studies; misclassification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Epidemiologie und Präventivmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Jul 2020 09:34 |
| Last Modified: | 13 Jul 2020 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24199 |
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