Roldan, J. C. and Detsch, R. and Schaefer, S. and Chang, E. and Kelantan, M. and Waiss, W. and Reichert, T. E. and Gurtner, G. C. and Deisinger, U. (2010) Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model. JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 38 (6). pp. 423-430. ISSN 1010-5182,
Full text not available from this repository. (Request a copy)Abstract
Introduction: Angiogenesis and mesenchymal stem cells (MSCs) promote osteogenesis. The aim of the present study was to evaluate whether bone morphogenetic protein (BMP-7) promoted osteoinduction could be enhanced by combining it with vascular endothelial growth factor (VEGF) or MSCs in highly porous biphasic calcium phosphate (BCP) ceramics. Materials and methods: BCP ceramic blocks were implanted in an ectopic site in 24 mice (BMP-7 vs. BMP-7/MSCs and BMP-7 vs. Control; each group n = 8). Specimens were analysed 12 weeks after surgery by environmental scanning electron microscopy (ESEM) and Giemsa staining. Results: In all implanted scaffolds, newly formed bone was observed, even in the control site. No statistical differences in the amount of new bone were found in the presence of BMP-7 compared to BMP-7/VEGF (p = 1.0) or BMP-7/MSCs (p = 0.786). ESEM revealed a degradation of the scaffolds. A higher degradation was observed in areas where no bone-implant contact was present compared to areas where the ceramic was integrated in newly formed bone. Conclusions: Neither VEGF nor MSCs enhanced BMP-7 induced bone formation under the selected conditions. The present ceramic seed to be osteoinductive and degradable, making this material suitable for bone tissue engineering. (C) European Association for Cranio-Maxillp-Facial Surgery.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OSTEOINDUCTIVE BIOMATERIALS; MORPHOGENETIC PROTEIN-2; GROWTH-FACTOR; REGENERATION; DEFECT; ANGIOGENESIS; CONSTRUCTS; SCAFFOLDS; RELEVANCE; DELIVERY; growth factors; BMP-7; VEGF; mesenchymal stem cells; calcium phosphate ceramic; ectopic bone formation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2020 06:01 |
| Last Modified: | 15 Jul 2020 06:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24255 |
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