Yang, Hua and Lipscomb, Gina L. and Keese, Annette M. and Schut, Gerrit J. and Thomm, Michael and Adams, Michael W. W. and Wang, Bi Cheng and Scott, Robert A. (2010) SurR regulates hydrogen production in Pyrococcus furiosus by a sulfur-dependent redox switch. MOLECULAR MICROBIOLOGY, 77 (5). pp. 1111-1122. ISSN 0950-382X, 1365-2958
Full text not available from this repository. (Request a copy)Abstract
P>We present structural and biochemical evidence for a redox switch in the archaeal transcriptional regulator SurR of Pyrococcus furiosus, a hyperthermophilic anaerobe. P. furiosus produces H-2 during fermentation, but undergoes a metabolic shift to produce H2S when elemental sulfur (S0) becomes available. Changes in gene expression occur within minutes of S0 addition, and the majority of these S0-responsive genes are regulatory targets of SurR, a key regulator involved in primary S0 response. SurR was shown in vitro to have dual functionality, activating transcription of some of these genes, notably the hydrogenase operons, and repressing others, including a gene-encoding sulfur reductase. This work demonstrates via biochemical and structural evidence that the activity of SurR is modulated by cysteine residues in a CxxC motif that constitutes a redox switch. Oxidation of the switch with S0 inhibits sequence-specific DNA binding by SurR, leading to deactivation of genes related to H-2 production and derepression of genes involved in S0 metabolism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN DISULFIDE OXIDOREDUCTASE; TRANSCRIPTIONAL REGULATOR; CRYSTAL-STRUCTURE; ELEMENTAL SULFUR; DENSITY MODIFICATION; ABC TRANSPORTER; RNA-POLYMERASE; SPECIFICITY; METABOLISM; REFINEMENT; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2020 06:54 |
| Last Modified: | 15 Jul 2020 06:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24271 |
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