Philippi, Anja and Steinbauer, Robert and Reiter, Alarich and Fath, Stephan and Leger-Silvestre, Isabelle and Milkereit, Philipp and Griesenbeck, Joachim and Tschochner, Herbert (2010) TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production. NUCLEIC ACIDS RESEARCH, 38 (16). pp. 5315-5326. ISSN 0305-1048,
Full text not available from this repository. (Request a copy)Abstract
Ribosome biogenesis is tightly linked to cellular growth. A crucial step in the regulation of ribosomal RNA (rRNA) gene transcription is the formation of the complex between RNA polymerase I (Pol I) and the Pol I-dependent transcription factor Rrn3p. We found that TOR inactivation leads to proteasome-dependent degradation of Rrn3p and a strong reduction in initiation competent Pol I-Rrn3p complexes affecting yeast rRNA gene transcription. Using a mutant expressing non-degradable Rrn3p or a strain in which defined endogenous Rrn3p levels can be adjusted by the Tet-off system, we can demonstrate that Rrn3p levels influence the number of Pol I-Rrn3p complexes and consequently rRNA gene transcription. However, our analysis reveals that the dramatic reduction of rRNA synthesis in the immediate cellular response to impaired TOR signalling cannot be explained by the simple down-regulation of Rrn3p and Pol I-Rrn3p levels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE-I; SACCHAROMYCES-CEREVISIAE; CHROMATIN STRUCTURES; GENE-EXPRESSION; TRANSCRIPTION; PROTEIN; PROTEASOME; PROMOTER; SYSTEM; GROWTH; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2020 07:16 |
| Last Modified: | 15 Jul 2020 07:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24282 |
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