Holtfreter, M. C. and Loebermann, M. and Frei, E. and Riebold, D. and Wolff, D. and Hartung, G. and Kinzelbach, R. and Reisinger, E. C. (2010) Schistosomula, pre-adults and adults of Schistosoma mansoni ingest fluorescence-labelled albumin in vitro and in vivo: implication for a drug-targeting model. PARASITOLOGY, 137 (11). pp. 1645-1652. ISSN 0031-1820, 1469-8161
Full text not available from this repository. (Request a copy)Abstract
Objective. Bilharziosis is one of the most important helminthal infections in humans and is caused by blood flukes of the genus Schistosoma. Three different life stages of the parasite occur within the mammalian host: schistosomula located in the skin, pre-adults located in the lung and adult worms located in the portal venous system. Erythrocytes are a major source of nutrient supply for adults. However, sources of nutrition for the developing stages are still unclear. Methods. To investigate whether schistosomula, pre-adults and adults of Schistosoma mansoni ingest human serum albumin (HSA) in vitro, these life stages were incubated with aminofluorescein-labelled human serum albumin (Afl-HSA) for 5 h. To test the uptake of albumin in vivo, the albumin conjugate was given intravenously to S. mansoni infected NMRI mice 24 h before harvesting the 3 life stages. Results. In comparison to the control group schistosomula, pre-adults, and adults showed an accumulation of Afl-HSA within the oesophagus and intestinal caecum in vitro and in vivo. Conclusion. Our findings suggest that albumin seems to be a major source of energy supply for the early schistosomal life stages and an additive energy support for adult worms. Since albumin has been used successfully as a drug carrier for chemotherapeutic substances against malignant disorders, further studies will focus on albumin as a carrier for anthelminthics in a drug-targeting model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER-SCANNING MICROSCOPY; TEXAS RED-BSA; EXCRETORY SYSTEM; METHOTREXATE-ALBUMIN; CELL CARCINOMA; SERUM-ALBUMIN; PHASE-II; MTX-HSA; DIGESTION; PARASITE; Schistosoma mansoni; schistosomula; pre-adults; adults; ingestion; human serum albumin; aminofluorescein; drug targeting |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2020 08:15 |
| Last Modified: | 15 Jul 2020 08:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24284 |
Actions (login required)
 |
View Item |
