Turel, Matejka and Duerkop, Axel and Yegorova, Alla and Karasyov, Alexander and Scripinets, Yulia and Lobnik, Aleksandra (2010) Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection. ANALYTICA CHIMICA ACTA, 675 (1). pp. 42-48. ISSN 0003-2670, 1873-4324
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We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3-100 mu mol L-1 range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the probe is quenched by both, primary (H2PO4-) and secondary phosphate (HPO42-). The LOD is 110 nmol L-1. A Stern-Volmer study revealed that quenching is mostly static. Due to the ms-decay time of TbL, the first luminescence lifetime assay for phosphate could also be developed. The lifetime-based calibration plot is linear between 0.5 and 5 mu mol L-1 of P. The effect of various surfactants on assay performance and a study on interferents are presented. The probe was successfully applied to determination of Pin commercial plant fertilizers and validated against the molybdenum blue test. The probe is the most sensitive lanthanide-based probe for phosphate. (C) 2010 Elsevier BM. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOSPHOANTIMONYLMOLYBDENUM BLUE COMPLEX; AQUEOUS-SOLUTION; EUROPIUM-TETRACYCLINE; ALKALINE-PHOSPHATASE; LANTHANIDE COMPLEXES; ANION RECOGNITION; PROBES; BINDING; COORDINATION; SELECTIVITY; Fluorescent probe; Lanthanide luminescence; Phosphate; Time-resolved spectroscopy; Microtiterplate assay; Lifetime assay |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2020 09:41 |
| Last Modified: | 15 Jul 2020 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24318 |
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