Beck, Hans Christian and Petersen, Jorgen and Nielsen, Soren Jensby and Morszeck, Christian and Jensen, Peter B. and Sehested, Maxwell and Grauslund, Morten (2010) Proteomic profiling of human colon cancer cells treated with the histone deacetylase inhibitor belinostat. ELECTROPHORESIS, 31 (16). pp. 2714-2721. ISSN 0173-0835,
Full text not available from this repository. (Request a copy)Abstract
The anticancer drug belinostat is a hydroxamate histone deacetylase inhibitor that has shown significant antitumour activity in various tumour models and also in clinical trials. In this study, we utilized a proteomic approach in order to evaluate the effect of this drug on protein expression in the human colon cancer cell line HCT116. Protein extracts from untreated HCT116 cells, and cells grown for 24 h in the presence of 1 and 10 mu M belinostat were analysed by 2-D gel electrophoresis. Proteins were visualized by colloidal Coomassie blue staining and quantitative analysis of gel images revealed 45 unique differentially expressed proteins that were identified by LC-MSMS analysis. Among these proteins, of particular interest are the downregulated proteins nucleophosmin and stratifin, and the upregulated proteins nucleolin, gelsolin, heterogeneous nuclear ribonucleoprotein K, annexin 1, and HSP90B that all were related to the protooncogene proteins p53, Myc, activator protein 1, and c-fos protein. The modulation of these proteins is consistent with the observations that belinostat is able to inhibit clonogenic cell growth of HCT116 cells and the biological role of these proteins will be discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HDAC INHIBITORS; TRICHOSTATIN-A; GENE-EXPRESSION; DOWN-REGULATION; P53; APOPTOSIS; LINES; NUCLEOPHOSMIN; PROTEINS; ACID; 2-D PAGE; Belinostat; Cancer treatment; HCT116; Histone deacetylase inhibitor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2020 08:25 |
| Last Modified: | 20 Jul 2020 08:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24370 |
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