Sasahira, Tomonori and Kirita, Tadaaki and Kurihara, Miyako and Yamamoto, Kazuhiko and Bhawal, Ujjal K. and Bosserhoff, Anja Katrin and Kuniyasu, Hiroki (2010) MIA-dependent angiogenesis and lymphangiogenesis are closely associated with progression, nodal metastasis and poor prognosis in tongue squamous cell carcinoma. EUROPEAN JOURNAL OF CANCER, 46 (12). pp. 2285-2294. ISSN 0959-8049, 1879-0852
Full text not available from this repository. (Request a copy)Abstract
We examined the role of angiogenesis/lymphangiogenesis and the relationship between melanoma inhibitory activity (MIA) and angiogenesis or lymphangiogenesis in oral squamous cell carcinoma (OSCC). One hundred and one formalin-fixed, paraffin-embedded specimens of primary OSCC were evaluated for microvessel density (MVD), lymphovessel density (LVD), expression of vascular endothelial growth factor (VEGF), VEGF-C, VEGF-D and MIA. Fresh frozen 18 samples of primary OSCC were further examined for the expression of VEGF, VEGF-C, VEGF-D and MIA protein by enzyme-linked immunosorbent assay (ELISA). In in vitro analysis, we studied the change of VEGF, VEGF-C and VEGF-D expression after MIA siRNA treatment. Higher MVD, LVD and VEGF expression levels were closely associated with tumour progression, nodal metastasis and poor prognosis. Expression levels of VEGF-C and VEGF-D were only related with nodal metastasis. MIA expression was significantly associated with MVD, LVD, VEGF, VEGF-C and VEGF-D expression by immunohistochemistry and ELISA assay. VEGF, VEGF-C, VEGF-D and MIA expression levels of metastatic tongue cancer HSC-3 cells were higher than those with no metastatic HSC-4 cells, and VEGF, VEGF-C and VEGF-D expression levels were decreased by MIA siRNA treatment in both cells. MIA-dependent angiogenesis/lymphangiogenesis might be a useful therapeutic target in progressive and metastatic OSCC. (C) 2010 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; GLYCATION END-PRODUCTS; MELANOMA INHIBITORY-ACTIVITY; BLOOD-VESSEL FORMATION; LYMPHATIC METASTASIS; VEGF-A; TUMOR LYMPHANGIOGENESIS; FACTOR EXPRESSION; GASTRIC-CANCER; BREAST-CANCER; Angiogenesis; Lymphangiogenesis; VEGF; VEGF-C; VEGF-D; MIA; OSCC |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2020 08:33 |
| Last Modified: | 20 Jul 2020 08:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24374 |
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