Maisch, Tim and Santarelli, Francesco and Schreml, Stephan and Babilas, Philipp and Szeimies, Rolf-Markus (2010) Fluorescence induction of protoporphyrin IX by a new 5-aminolevulinic acid nanoemulsion used for photodynamic therapy in a full-thickness ex vivo skin model. EXPERIMENTAL DERMATOLOGY, 19 (8). E302-E305. ISSN 0906-6705,
Full text not available from this repository. (Request a copy)Abstract
An ex vivo porcine skin model was utilized to analyse the penetration of 5-aminolevulinic acid (5-ALA) contained in a nanoemulsion-based formulation BF-200 ALA (10% 5-ALA-hydrochloride) versus 16% aminolevulinate methyl ester-hydrochloride in a commercially cream (MAL cream) by fluorescence microscopy of their common metabolite protoporphyrin IX (PpIX) after 3, 5, 8 and 12 h. Fluorescence signals of PpIX in pig skin treated with BF-200 ALA were stronger than those for MAL cream. At 8 and 12 h, the PpIX fluorescence signals were 4.8- and 5.0-fold higher than those measured after MAL cream application. Fluorescence signals of PpIX after application of BF-200 ALA were detected in deeper tissue layers of the epidermis than after application of MAL cream (97.2 +/- 5.7 mu m for BF-200 ALA vs 42.0 +/- 4.2 mu m for MAL cream). These data implicate that BF-200 ALA in photodynamic therapy might lead to a superior therapeutically effect of intraepidermal (in situ) squamous cell carcinomas.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LECITHIN MICROEMULSION GEL; HUMAN STRATUM-CORNEUM; TRANSDERMAL TRANSPORT; PENETRATION; LIPOSOMES; DELIVERY; distribution; nanoemulsion; penetration enhancer; photodynamic; porcine skin; stratum corneum |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jul 2020 08:46 |
| Last Modified: | 20 Jul 2020 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24382 |
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