Kubosaki, Atsutaka and Lindgren, Gabriella and Tagami, Michihira and Simon, Christophe and Tomaru, Yasuhiro and Miura, Hisashi and Suzuki, Takahiro and Arner, Erik and Forrest, Alistair R. R. and Irvine, Katharine M. and Schroder, Kate and Hasegawa, Yuki and Kanamori-Katayama, Mutsumi and Rehli, Michael and Hume, David A. and Kawai, Jun and Suzuki, Masanori and Suzuki, Harukazu and Hayashizaki, Yoshihide (2010) The combination of gene perturbation assay and ChIP-chip reveals functional direct target genes for IRF8 in THP-1 cells. MOLECULAR IMMUNOLOGY, 47 (14). pp. 2295-2302. ISSN 0161-5890,
Full text not available from this repository.Abstract
Gene regulatory networks in living cells are controlled by the interaction of multiple cell type-specific transcription regulators with DNA binding sites in target genes Interferon regulatory factor 8 (IRF8) also known as Interferon consensus sequence binding protein (ICSBP) is a transcription factor expressed predominantly in myeloid and lymphoid cell lineages To find the functional direct target genes of IRF8 the gene expression profiles of siRNA knockdown samples and genome-wide binding locations by ChIP-chip were analyzed in THP-1 myelomonocytic leukemia cells Consequently 84 genes were identified as functional direct targets The ETS family transcription factor PU 1 also known as SPI1 binds to IRF8 and regulates basal transcription in macrophages Using the same approach we identified 53 direct target genes of PU 1 these overlapped with 19 IRF8 targets These 19 genes included key molecules of IFN signaling such as OAS1 and IRF9 but excluded other IFN-related genes amongst the IRF8 functional direct target genes We suggest that IRF8 and PU 1 can have both combined and independent actions on different promoters in myeloid cells (C) 2010 Elsevier Ltd All rights reserved
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SEQUENCE-BINDING-PROTEIN; REGULATORY FACTOR FAMILY; TRANSCRIPTION FACTORS; MYELOID CELLS; INTERFERON; PU.1; MACROPHAGES; DIFFERENTIATION; EXPRESSION; MOUSE; ChIp-chip; IRF8; PU 1; Interferon |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Jul 2020 12:14 |
| Last Modified: | 21 Jul 2020 12:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24411 |
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