Thoelking, Gerold and Reiss, Bjoern and Wegener, Joachim and Oberleithner, Hans and Pavenstaedt, Hermann and Riethmuller, Christoph (2010) Nanotopography follows force in TGF-beta 1 stimulated epithelium. NANOTECHNOLOGY, 21 (26): 265102. ISSN 0957-4484,
Full text not available from this repository. (Request a copy)Abstract
Inflammation and cellular fibrosis often imply an involvement of the cytokine TGF-beta 1. TGF-beta 1 induces epithelial-to-mesenchymal transdifferentiation (EMT), a term describing the loss of epithelium-specific function. Indicative for this process are an elongated cell shape parallel to stress fibre formation. Many signalling pathways of TGF-beta 1 have been discovered, but mechanical aspects have not yet been investigated. In this study, atomic force microscopy (AFM) was used to analyse surface topography and mechanical properties of EMT in proximal kidney tubule epithelium (NRK52E). Elongated cells, an increase of stress fibre formation and a loss of microvillus compatible structures were observed as characteristic signs of EMT. Furthermore, AFM could identify an increase in stiffness by 71% after six days of stimulation with TGF-beta 1. As a novel topographical phenomenon, nodular protrusions emerged at the cell-cell junctions. They occurred preferentially at sites where stress fibres cross the border. Since these nodular protrusions were sensitive to inhibitors of force generation, they can indicate intracellular tension. The results demonstrate a manifest impact of elevated tension on the cellular topography.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL TRANSITION; ENDOTHELIAL-CELLS; MICROSCOPY; TRANSDIFFERENTIATION; PROTEINS; ELASTICITY; MOLECULES; VOLUME; SHAPE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2020 07:17 |
| Last Modified: | 27 Jul 2020 07:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24470 |
Actions (login required)
 |
View Item |
