Dettmer, Katja and Almstetter, Martin F. and Appel, Inka J. and Nuernberger, Nadine and Schlamberger, Gregor and Gronwald, Wolfram and Meyer, Heinrich H. D. and Oefner, Peter J. (2010) Comparison of serum versus plasma collection in gas chromatography - Mass spectrometry-based metabolomics. ELECTROPHORESIS, 31 (14). pp. 2365-2373. ISSN 0173-0835, 1522-2683
Full text not available from this repository. (Request a copy)Abstract
Bovine serum, EDTA-plasma and EDTA-plasma fortified with acetylsalicylic acid (ASA) as antioxidant were compared with regard to their suitability for metabolomic studies. Metabolic fingerprints were generated from GC-TOF-MS data using the Leco Chroma-TOF (R) software in combination with the in-house retention time correction and data alignment tool INCA. A total of 6, 9 and 21 significant features with a false discovery rate of <0.05 were identified by INCA upon comparing EDTA- versus EDTA-ASA-plasma, EDTA-plasma versus serum and EDTA-ASA-plasma versus serum, respectively. To confirm that the observed signal intensities in the GC-TOF-MS fingerprints reflected true metabolite abundances, 19 amino acids, glucose and 6 organic acids were quantified by means of GC-MS using stable-isotope-labeled internal standards. As observed with the fingerprints, only the concentrations of lactate and citrate were found to be significantly lower in EDTA-plasma and serum, respectively, whereas the concentrations of the other metabolites were similar among the three sample types investigated.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLYCOLYSIS; DISCOVERY; GLUCOSE; IMPACT; BIAS; Blood plasma; GC-MS; Metabolomics; Organic acids; Serum |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2020 08:40 |
| Last Modified: | 27 Jul 2020 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24492 |
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