Graessel, Susanne and Beckmann, Johannes and Rath, Bjoern and Vogel, Mandy and Grifka, Joachim and Tingart, Markus (2010) Expression profile of matrix metalloproteinase-2 and-9 and their endogenous tissue inhibitors in osteonecrotic femoral heads. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 26 (1). pp. 127-133. ISSN 1107-3756,
Full text not available from this repository. (Request a copy)Abstract
The pathogenetic mechanisms of osteonecrosis of the femoral head are unresolved to date. This study analyzed the matrix metalloprotease (MMP) and tissue inhibitor of matrix metalloprotease (TIMP) expression and activity which might add to the impaired bone matrix repair capacity affecting the balance between bone resorption and de 1101,0 bone formation in osteonecrosis of the femoral head (ONFH). Cancellous bone biopsies were taken at the femoral head and neck of patients with advanced ONFH and patients with primary osteoarthritis (OA) who were undergoing total hip arthroplasty. We assessed the gene expression levels of MMP-2 and -9. TIMP-1 and -2, IL-1 beta, IL-6 and TNF-alpha in both biopsies. These data were corroborated by MMP activity screening, collagen profiling and ELISAs for determination of MMP. TIMP. IL-1 beta, IL-6 and TNF-alpha concentrations. Gene expression rates of MMP-2 and TIMP-1 were higher in ONFH biopsies whereas TIMP-2 and MMP-9 gene expression was not significantly altered. MMP protein profile shifted towards increased biosynthesis of both, active and pro-MMP-2 in ONFH bone lysates and decreased pro-MMP-9 production. Expression profiles of pro-inflammatory cytokines and collagens in OA and ONFH bone lysates were highly similar. Increased biosynthesis and activation of MMP-2 in ONFH samples may add to shifting the bone matrix turnover balance towards resorption of bone macromolecules thereby counteracting new bone matrix production. OA- and ONFH-affected bone exhibits a similar pro-inflammatory cytokine and collagen expression profile suggesting a relationship on the molecular level of inflammation and collagenous matrix composition between both diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; BONE-RESORPTION; ARTICULAR CHONDROCYTES; EXTRACELLULAR-MATRIX; OSTEOBLASTIC CELLS; PROTEIN-C; MMP-9; MATRIX-METALLOPROTEINASE-9; DIFFERENTIATION; INTERLEUKIN-1; osteonecrosis; osteoarthritis; matrix metalloproteinase; tissue inhibitor matrix metalloprotease; collagen |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2020 10:35 |
| Last Modified: | 27 Jul 2020 10:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24508 |
Actions (login required)
 |
View Item |
