Sasaki, Takako and Takagi, Junichi and Giudici, Camilla and Yamada, Yoshihiko and Arikawa-Hirasawa, Eri and Deutzmann, Rainer and Timpl, Rupert and Sonnenberg, Arnoud and Baechinger, Hans Peter and Tonge, David (2010) Laminin-121-Recombinant expression and interactions with integrins. MATRIX BIOLOGY, 29 (6). pp. 484-493. ISSN 0945-053X,
Full text not available from this repository. (Request a copy)Abstract
Laminin-121. previously referred as to laminin-3, was expressed recombinantly in human embryonic kidney (HEK) 293 cells by triple transfection of full-length cDNAs encoding mouse laminin alpha 1, beta 2 and gamma 1 chains. The recombinant laminin-121 was purified using Heparin-Sepharose followed by molecular sieve chromatography and shown to be correctly folded by electron microscopy and circular dichroism (CD). The CD spectra of recombinant laminin-121 were very similar to those of laminin-111 isolated from Engelbreth-Holm-Swarm tumor (EHS-laminin) but its T(m) value was smaller than EHS-laminin and recombinant lamnin-111 suggesting that the replacement of the beta chain reduced the stability of the coiled-coil structure of laminin-121. Its binding to integrins was compared with EHS-laminin, laminin-3A32 purified from murine epidermal cell line and recombinantly expressed laminins-111, -211 and -221. Laminin-121 showed the highest affinity to alpha 6 beta 1 and alpha 7 beta 1 integrins and furthermore, laminin-121 most effectively supported neurite outgrowth. Together, this suggests that the beta 2 laminins have higher affinity for integrins than the beta 1 laminins. Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALPHA-DYSTROGLYCAN BINDING; LAMININ LAMININ BETA-2; BASEMENT-MEMBRANES; NEURITE OUTGROWTH; CELL-ADHESION; S-LAMININ; EXTRACELLULAR-MATRIX; HUMAN PLACENTA; LG MODULES; IN-VIVO; Extracellular matrix; Basement membrane; Laminin; Recombinant expression; Integrin; Neurite outgrowth |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Jul 2020 12:15 |
| Last Modified: | 27 Jul 2020 12:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24522 |
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