Xu, Ling and Hausmann, Martin and Dietmaier, Wolfgang and Kellermeier, Silvia and Pesch, Theresa and Stieber-Gunckel, Manuela and Lippert, Elisabeth and Klebl, Frank and Rogler, Gerhard (2010) Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines. BMC CANCER, 10: 302. ISSN 1471-2407,
Full text not available from this repository. (Request a copy)Abstract
Background: Cholangiocarcinoma (CC) is a malignant neoplasm of the bile ducts or the gallbladder. Targeting of growth factor receptors showed therapeutic potential in palliative settings for many solid tumors. The aim of this study was to determine the expression of seven growth factor receptors in CC cell lines and to assess the effect of blocking the EGFR receptor in vitro. Methods: Expression of EGFR (epithelial growth factor receptor), HGFR (hepatocyte growth factor receptor) IGF1R (insulin-like growth factor 1 receptor), IGF2R (insulin-like growth factor 2 receptor) and VEGFR1-3 (vascular endothelial growth factor receptor 1-3) were examined in four human CC cell lines (EGI-1, HuH28, OZ and TFK-1). The effect of the anti-EGFR-antibody cetuximab on cell growth and apoptosis was studied and cell lines were examined for KRAS mutations. Results: EGFR, HGFR and IGFR1 were present in all four cell lines tested. IGFR2 expression was confirmed in EGI-1 and TFK-1. No growth-inhibitory effect was found in EGI-1 cells after incubation with cetuximab. Cetuximab dose-dependently inhibited growth in TFK-1. Increased apoptosis was only seen in TFK-1 cells at the highest cetuximab dose tested (1 mg/ml), with no dose-response-relationship at lower concentrations. In EGI-1 a heterozygous KRAS mutation was found in codon 12 (c.35G>A; p.G12D). HuH28, OZ and TFK-1 lacked KRAS mutation. Conclusion: CC cell lines express a pattern of different growth receptors in vitro. Growth factor inhibitor treatment could be affected from the KRAS genotype in CC. The expression of EGFR itself does not allow prognoses on growth inhibition by cetuximab.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UP-REGULATES P27(KIP1); MONOCLONAL-ANTIBODY; CHOLANGIOCELLULAR CARCINOMA; COLORECTAL-CANCER; TUMOR-GROWTH; NUDE-MICE; INHIBITION; CETUXIMAB; INSULIN; APOPTOSIS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Jul 2020 06:40 |
| Last Modified: | 28 Jul 2020 06:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24554 |
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