El Chartouni, Carol and Benner, Chris and Eigner, Monika and Lichtinger, Monika and Rehli, Michael (2010) Transcriptional effects of Colony-stimulating factor-1 in mouse macrophages. IMMUNOBIOLOGY, 215 (6). pp. 466-474. ISSN 0171-2985,
Full text not available from this repository. (Request a copy)Abstract
Colony-stimulating factor-1 (CSF-1) is a major regulator of macrophage development. CSF-1-dependent signalling has been implicated in proliferation, survival, and differentiation of mononuclear phagocytes, however, relatively little is known about the effects of CSF-1 on macrophage gene transcription and on CSF-1-responsive gene promoters. We used a combination of transcription profiling and in silico motif search to characterize genes that are regulated in mature bone marrow-derived macrophages cultured in the presence or absence of CSF-1. The expression of many known differentiation-associated macrophage markers was not significantly affected in the absence of CSF-1. Genes repressed by CSF-1 comprised a considerable number of granulocyte-specific genes. The respective gene promoters; however, were not significantly enriched for specific DNA patterns, suggesting that these genes are regulated by promoter-distal elements or at a post-transcriptional level. Genes downregulated upon CSF-1 deprivation showed a highly significant association with cell division which is in line with the known role of CSF-1 as a proliferation stimulus for mouse macrophages. Interestingly, three DNA patterns were significantly co-enriched in CSF-1-dependent gene promoters, including motifs related to NFY, CHR, and E2F sites. These motifs showed a strong positional preference on target promoters at 60, 30 and 0 bp upstream of the transcription start site, and define the common promoter structure of CSF-1-responsive genes. (C) 2009 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED PROTEIN-KINASE; CELL-CYCLE; NF-Y; NEUTROPHIL GELATINASE; HEMATOPOIETIC-CELLS; GRANULE PROTEIN; EARLY EVENTS; M-CSF; EXPRESSION; PROLIFERATION; Macrophage colony-stimulating factor; Gene regulation; Transcription factors |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2020 06:44 |
| Last Modified: | 29 Jul 2020 06:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24620 |
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