Erlach, Markus Beck and Munte, Claudia E. and Kremer, Werner and Hartl, Rainer and Rochelt, Doerte and Niesner, Dieter and Kalbitzer, Hans Robert (2010) Ceramic cells for high pressure NMR spectroscopy of proteins. JOURNAL OF MAGNETIC RESONANCE, 204 (2). pp. 196-199. ISSN 1090-7807, 1096-0856
Full text not available from this repository. (Request a copy)Abstract
Application of high pressure to biological macromolecules can be used to find new structural states with a smaller specific volume of the system. High pressure NMR spectroscopy is a most promising analytical tool for the study of these states at atomic resolution. High pressure quartz cells are difficult to handle, high quality sapphire high pressure cells are difficult to obtain commercially. In this work, we describe the use of high pressure ceramic cells produced from yttrium stabilized ZrO2 that are capable of resisting pressures up to 200 MPa. Since the new cells should also be usable in the easily damageable cryoprobes a completely new autoclave for these cells has been constructed, including an improved method for pressure transmission, an integrated safety jacket, a displacement body, and a fast self-closing emergency valve. (C) 2010 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; HUMAN PRION PROTEIN; HIGH-RESOLUTION; STATES; High pressure; NMR spectroscopy; Ceramic cell; Proteins |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2020 08:53 |
| Last Modified: | 29 Jul 2020 08:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24640 |
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