Vollmann-Zwerenz, Arabel and Diermeier-Daucher, Simone and Wege, Anja Kathrin and Sassen, Andrea and Schmidt-Bruecken, Elisabeth and Hofstaedter, Ferdinand and Ortmann, Olaf and Nauwelaers, Frans and Brockhoff, Gero (2010) Multichromatic Phenotyping of HER Receptor Coexpression in Breast Tumor Tissue Samples Using Flow Cytometry-Possibilities and Limitations. CYTOMETRY PART A, 77A (4). pp. 387-398. ISSN 1552-4922, 1552-4930
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The prognostic significance of HER2 expression in human breast carcinomas is beyond dispute nowadays. The HER family of receptor tyrosine kinases comprises four members (HER1/ErbB1/EGFR, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4) that act in concert via transactivation and consequently compose a functional signaling unit. Besides HER2 overexpression, coexpression of other HER receptors has substantial impact on course of disease and potential therapeutic benefit. This observation is substantiated by numerous preclinical studies and retrospective studies done on patients with breast cancer. Against this background, the quantification of all HER receptor expressions at the same time would significantly extend the information content revealed by routine diagnosis of breast cancer tissues. Moreover, the knowledge of HER receptor coexpression profiles in primary tumor samples could provide the basis to design and develop highly specific antireceptor treatment strategies. Here, we report on a simultaneous flow cytometric detection of all four HER receptors on carcinoma cells isolated from primary breast cancer tissues and separated from nonepithelial cells by cytokeratin staining. Combined with DNA, i.e. ploidy quantification, the approach resulted in a six-parameter assay that could complement the diagnosis of a variety of diseases in which HER receptor expression has a pivotal impact on the degree of malignancy. (C) 2010 International Society for Advancement of Cytometry
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR RECEPTOR; 2 CELLULAR ANTIGENS; DNA-PLOIDY; PROGNOSTIC VALUE; PROPIDIUM-IODIDE; CANCER PATIENTS; EGFR FAMILY; CARCINOMAS; EXPRESSION; CELLS; receptor tyrosine kinases; multiparametric flow cytometry; solid tumor dissociation; DNA assessment |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Aug 2020 08:25 |
| Last Modified: | 03 Aug 2020 08:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24862 |
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