Gruenberg, Sebastian and Reich, Christoph and Zeller, Mirijam E. and Bartlett, Michael S. and Thomm, Michael (2010) Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes. NUCLEIC ACIDS RESEARCH, 38 (6). pp. 1950-1963. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
The lower jaws of archaeal RNA polymerase and eukaryotic RNA polymerase II include orthologous subunits H and Rpb5, respectively. The tertiary structure of H is very similar to the structure of the C-terminal domain of Rpb5, and both subunits are proximal to downstream DNA in pre-initiation complexes. Analyses of reconstituted euryarchaeal polymerase lacking subunit H revealed that H is important for open complex formation and initial transcription. Eukaryotic Rpb5 rescues activity of the delta H enzyme indicating a strong conservation of function for this subunit from archaea to eukaryotes. Photochemical cross-linking in elongation complexes revealed a striking structural rearrangement of RNA polymerase, bringing subunit H near the transcribed DNA strand one helical turn downstream of the active center, in contrast to the positioning observed in preinitiation complexes. The rearrangement of subunits H and A'' suggest a major conformational change in the archaeal RNAP lower jaw upon formation of the elongation complex.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION INITIATION COMPLEX; STRUCTURAL BASIS; PREINITIATION COMPLEX; TERNARY COMPLEXES; CLEAVAGE ACTIVITY; TFIIE-ALPHA; DNA HYBRID; FACTOR-SII; TATA-BOX; TRANSITION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Aug 2020 12:09 |
| Last Modified: | 04 Aug 2020 12:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24943 |
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