Kalamajka, Rainer and Finnie, Christine and Grasser, Klaus D. (2010) Onset of grain filling is associated with a change in properties of linker histone variants in maize kernels. PLANTA, 231 (5). pp. 1127-1135. ISSN 0032-0935, 1432-2048
Full text not available from this repository. (Request a copy)Abstract
In maize kernel development, the onset of grain-filling represents a major developmental switch that correlates with a massive reprogramming of gene expression. We have isolated chromosomal linker histones from developing maize kernels before (11 days after pollination, dap) and after (16 dap) initiation of storage synthesis. Six linker histone gene products were identified by MALDI-TOF mass spectrometry. A marked shift of around 4 pH units was observed for the linker histone spot pattern after 2D-gel electrophoresis when comparing the proteins of 11 and 16 dap kernels. The shift from acidic to more basic protein forms suggests a reduction in the level of post-translational modifications of linker histones during kernel development. Analysis of their DNA-binding affinity revealed that the different linker histone gene products bind double-stranded DNA with similar affinity. Interestingly, the linker histones isolated from 16 dap kernels consistently displayed a lower affinity for DNA than the proteins isolated from 11 dap kernels. These findings suggest that the affinity for DNA of the linker histones may be regulated by post-translational modification and that the reduction in DNA affinity could be involved in a more open chromatin during storage synthesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARABIDOPSIS-THALIANA; ENDOSPERM DEVELOPMENT; PROTEOME ANALYSIS; GENETIC-ANALYSIS; H1 VARIANTS; HMG DOMAIN; CHROMATIN; EXPRESSION; PROTEINS; DNA; Chromatin; DNA interaction; H1; Linker histone; 2D-gel electrophoresis |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Aug 2020 09:58 |
| Last Modified: | 03 Aug 2020 09:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24952 |
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