Sparmann, Gisela and Kruse, Marie-Luise and Hofmeister-Mielke, Nicole and Koczan, Dirk and Jaster, Robert and Liebe, Stefan and Wolff, Daniel and Emmrich, Joerg (2010) Bone marrow-derived pancreatic stellate cells in rats. CELL RESEARCH, 20 (3). pp. 288-298. ISSN 1001-0602,
Full text not available from this repository. (Request a copy)Abstract
Origin and fate of pancreatic stellate cells (PSCs) before, during and after pancreatic injury are a matter of debate. The crucial role of PSCs in the pathogenesis of pancreatic fibrosis is generally accepted. However, the turnover of the cells remains obscure. The present study addressed the issue of a potential bone marrow (BM) origin of PSCs. We used a model of stable hematopoietic chimerism by grafting enhanced green fluorescence protein (eGFP)-expressing BM cells after irradiation of acceptor rats. Chimerism was detected by FACS analysis of eGFP-positive cells in the peripheral blood. Dibutyltin dichloride (DBTC) was used to induce acute pancreatic inflammation with subsequent recovery over 4 weeks. Investigations have been focused on isolated cells to detect the resting PSC population. The incidence of eGFP-positive PSC obtained from the pancreas of chimeric rats was approximately 7% in healthy pancreatic tissue and increased significantly to a mean of 18% in the restored pancreas 4 weeks after DBTC-induced acute inflammation. Our results suggest that BM-derived progenitor cells represent a source of renewable stellate cells in the pancreas. Increased numbers of resting PSCs after regeneration point toward enhanced recruitment of BM-derived cells to the pancreas and/or re-acquisition of a quiescent state after inflammation-induced activation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STEM-CELLS; DIBUTYLTIN DICHLORIDE; FIBROSIS; IDENTIFICATION; DIFFERENTIATION; REGENERATION; FIBROCYTES; APOPTOSIS; CULTURE; bone marrow; rat; pancreas; regeneration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2020 04:44 |
| Last Modified: | 05 Aug 2020 04:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25018 |
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