Paragh, Gyoergy and Ugocsai, Peter and Vogt, Thomas and Schling, Petra and Kel, Alexander E. and Tarabin, Victoria and Liebisch, Gerhard and Orso, Evelyn and Marko, Lorant and Balogh, Attila and Koebling, Tamas and Remenyik, Eva and Wikonkal, Norbert M. and Mandl, Jozsef and Farwick, Mike and Schmitz, Gerd (2010) Whole genome transcriptional profiling identifies novel differentiation regulated genes in keratinocytes. EXPERIMENTAL DERMATOLOGY, 19 (3). pp. 297-301. ISSN 0906-6705, 1600-0625
Full text not available from this repository. (Request a copy)Abstract
Keratinocyte differentiation plays a pivotal role in the epidermal barrier. Single keratinocyte differentiation genes have already been studied, but many important constituents of this process may have been missed so far. Gene expression profiling by microarray was carried out in cultured normal human epidermal keratinocytes undergoing confluence-induced differentiation to find novel differentiation genes. Candidate gene lists were established and genes of potential dermatological interest were validated by quantitative reverse transcription polymerase chain reaction and immunohistochemical analysis. Some of these points lead to the identification of counter-regulation of heme oxygenase and biliverdin reductase as well as glutaredoxin and glutathione reductase indicative of potential novel redox signaling in differentiating human keratinocytes. Others indicate a strong concert down-regulation of interleukin-1 signaling at previously unidentified levels during keratinocyte differentiation. We believe that identified genes contribute to a more comprehensive understanding of the complicated epidermal differentiation process and lead to better understanding of dermatological diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IL-1 RECEPTOR ANTAGONIST; SKIN BARRIER; EXPRESSION; THIOREDOXIN; ICHTHYOSIS; REDUCTASE; MOUSE; CELL; PCR; TPA; differentiation; glutaredoxin; heme oxygenase; interleukin-1; keratinocyte |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2020 06:33 |
| Last Modified: | 05 Aug 2020 06:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25071 |
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