Razny, Urszula and Polus, Anna and Kiec-Wilk, Beata and Wator, Lukasz and Hartwich, Jadwiga and Stachura, Jerzy and Tomaszewska, Romana and Dyduch, Grzegorz and Laidler, Piotr and Schmitz, Gerd and Goralczyk, Regina and Wertz, Karin and Riss, George and Franssen-van Hal, Nicole L. W. and Keijer, Jaap and Dembinska-Kiec, Aldona (2010) Angiogenesis in Balb/c mice under beta-carotene supplementation in diet. GENES AND NUTRITION, 5 (1). pp. 9-16. ISSN 1555-8932,
Full text not available from this repository. (Request a copy)Abstract
Angiogenesis is a process of new blood vessel formation from pre-existing ones. The most important steps in angiogenesis include detachment, proliferation, migration, homing and differentiation of vascular wall cells, which are mainly endothelial cells and their progenitors. The study focused on the effect of beta-carotene (BC) supplementation (12,000 mg/kg) in the diet on angiogenesis in Balb/c mice. Female Balb/c mice were fed for 5 weeks with two different diets: with BC or without BC supplementation. After 4 weeks of feeding, Balb/c mice were injected subcutaneously with two matrigel plugs with or without basic fibroblast growth factor (bFGF). Six days later, the animals were killed, and the matrigel plugs were used for immunohistochemical staining with CD31 antibody and for gene expression analysis. Microarray and Real-Time PCR data showed down-regulation of genes involved in proliferation and up-regulation of genes encoding inhibitors of apoptosis, proteins regulating cell adhesion, matrix-degrading enzymes and proteins involved in the VEGF pathway. The results of this study demonstrated that BC proangiogenic activity (with or without bFGF) in vivo seemed to be more significantly associated with cells' protection from apoptosis and their stimulation of chemotaxis/homing than cell proliferation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHEMOTAXIS; ACTIVATION; Angiogenesis; Beta-carotene; Chemotaxis; Microarray |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2020 06:26 |
| Last Modified: | 05 Aug 2020 06:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25072 |
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