Oppermann, Mona and Gess, Bernhard and Schweda, Frank and Castrop, Hayo (2010) Atrap Deficiency Increases Arterial Blood Pressure and Plasma Volume. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 21 (3). pp. 468-477. ISSN 1046-6673, 1533-3450
Full text not available from this repository. (Request a copy)Abstract
The angiotensin receptor-associated protein (Atrap) interacts with angiotensin II (Angll) type I (AT1) receptors and facilitates their internalization in vitro, but little is known about the function of Atrap in vivo. Here, we detected Atrap expression in several organs of wild-type mice; the highest expression was in the kidney where it localized to the proximal tubule, particularly the brush border. There was no Atrap expression in the renal vasculature or juxtaglomerular cells. We generated Atrap-deficient (Atrap-/-) mice, which were viable and seemed grossly normal. Mean systolic BP was significantly higher in Atrap-/- mice compared with wild-type mice. Dose-response relationships of arterial BP after acute Angll infusion were similar in both genotypes. Plasma volume was significantly higher and plasma renin concentration was markedly lower in Atrap-/- mice compared with wild-type mice. I-125-Angll binding showed enhanced surface expression of AT1 receptors in the renal cortex of Atrap-/- mice, accompanied by increased carboanhydrase-sensitive proximal tubular function. In summary, Atrap-/- mice have increased arterial pressure and plasma volume. Atrap seems to modulate volume status by acting as a negative regulator of AT1 receptors in the renal tubules.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANGIOTENSIN-II RECEPTOR; TRUNCATED AT(1A) RECEPTOR; MUSCLE-CELL-GROWTH; TYPE-1 RECEPTOR; GENE-EXPRESSION; INTERACTING MOLECULE; SIGNAL-TRANSDUCTION; RENIN SECRETION; AT1 RECEPTOR; RAT-KIDNEY; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kinder- und Jugendmedizin Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Aug 2020 09:32 |
| Last Modified: | 05 Aug 2020 09:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25095 |
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