Nemeth, Attila and Conesa, Ana and Santoyo-Lopez, Javier and Medina, Ignacio and Montaner, David and Peterfia, Balint and Solovei, Irina and Cremer, Thomas and Dopazo, Joaquin and Laengst, Gernot (2010) Initial Genomics of the Human Nucleolus. PLOS GENETICS, 6 (3): e1000889. ISSN 1553-7404,
Full text not available from this repository. (Request a copy)Abstract
We report for the first time the genomics of a nuclear compartment of the eukaryotic cell. 454 sequencing and microarray analysis revealed the pattern of nucleolus-associated chromatin domains (NADs) in the linear human genome and identified different gene families and certain satellite repeats as the major building blocks of NADs, which constitute about 4% of the genome. Bioinformatic evaluation showed that NAD-localized genes take part in specific biological processes, like the response to other organisms, odor perception, and tissue development. 3D FISH and immunofluorescence experiments illustrated the spatial distribution of NAD-specific chromatin within interphase nuclei and its alteration upon transcriptional changes. Altogether, our findings describe the nature of DNA sequences associated with the human nucleolus and provide insights into the function of the nucleolus in genome organization and establishment of nuclear architecture.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-III; HUMAN-CELLS; ACROCENTRIC CHROMOSOMES; SEGMENTAL DUPLICATIONS; INTERPHASE NUCLEI; GENE-EXPRESSION; RIBOSOMAL-RNA; CHROMATIN; ORGANIZATION; TRANSCRIPTION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Aug 2020 05:20 |
| Last Modified: | 06 Aug 2020 05:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25131 |
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