Winkelmann, Nadine and Jaekel, Ulrike and Meyer, Carolin and Serrano, Wilbert and Rachel, Reinhard and Rossello-Mora, Ramon and Harder, Jens (2010) Determination of the Diversity of Rhodopirellula Isolates from European Seas by Multilocus Sequence Analysis. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76 (3). pp. 776-785. ISSN 0099-2240, 1098-5336
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In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-TARGETED PROBES; CHLAMYDIA-TRACHOMATIS; GENUS PIRELLULA; COMB. NOV; PLANCTOMYCETE; STRAINS; IDENTIFICATION; BIOGEOGRAPHY; POPULATIONS; ULTRASTRUCTURE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Aug 2020 09:27 |
| Last Modified: | 07 Aug 2020 09:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25170 |
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