Kotz, Andrea and Wagener, Johannes and Engel, Jakob and Routier, Francoise and Echtenacher, Bernd and Pich, Andreas and Rohde, Manfred and Hoffmann, Petra and Heesemann, Juergen and Ebel, Frank (2010) The mitA gene of Aspergillus fumigatus is required for mannosylation of inositol-phosphorylceramide, but is dispensable for pathogenicity. FUNGAL GENETICS AND BIOLOGY, 47 (2). pp. 169-178. ISSN 1087-1845, 1096-0937
Full text not available from this repository. (Request a copy)Abstract
GDP-mannose:inositol-phosphorylceramide (MIPC)-derived glycosphingolipids are important pathogen-associated molecular patterns (PAMP) of Candida albicans and according to recently published data also of Aspergillus fumigatus. MIPC transferases are essential for the synthesis of MIPC, but have so far been studied only in Saccharomyces cerevisiae and C. albicans. Here, we have identified MitA as the only MIPC transferase in A. fumigatus. The Delta mitA mutant lacks MIPC and MIPC-derived glycosphingolipids and accumulates the precursor IPC. The mutant grows normally, shows no defects in cell wall or membrane organization and a normal resistance to different stressors. It is, however, sensitive to high Ca2+ concentrations, especially during germination. Germination of Delta mitA mutant conidia is also decelerated under normal growth conditions, but neither the virulence of this mutant in a systemic model of infection nor its ability to trigger a cytokine response in macrophages is impaired, arguing against a role of MIPC-derived glycosphingolipids as important A. fumigatus PAMPs. (c) 2009 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANDIDA-ALBICANS PHOSPHOLIPOMANNAN; SACCHAROMYCES-CEREVISIAE; PROTEIN; YEAST; VIRULENCE; GROWTH; STARVATION; VIABILITY; NIDULANS; POLARITY; Aspergillus fumigatus; Glycosphingolipids; MIPC; Innate immune response; Mannosylation; Germination |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Aug 2020 11:53 |
| Last Modified: | 07 Aug 2020 11:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25208 |
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