Svensson, Marcus and Russell, Karen and Mack, Matthias and Else, Kathryn J. (2010) CD4+T-cell localization to the large intestinal mucosa during Trichuris muris infection is mediated by G alpha(i)-coupled receptors but is CCR6-and CXCR3-independent. IMMUNOLOGY, 129 (2). pp. 257-267. ISSN 0019-2805, 1365-2567
Full text not available from this repository. (Request a copy)Abstract
P>Infection of mice with the gastrointestinal nematode Trichuris muris represents a valuable tool to investigate and dissect intestinal immune responses. Resistant mouse strains respond to T. muris infection by mounting a T helper type 2 response. Previous results have shown that CD4+ T cells play a critical role in protective immunity, and that CD4+ T cells localize to the infected large intestinal mucosa to confer protection. Further, transfer of CD4+ T cells from immune mice to immunodeficient SCID mice can prevent the development of a chronic infection. In the current study, we characterize the protective CD4+ T cells, describe their chemokine receptor expression and explore the functional significance of these receptors in recruitment to the large intestinal mucosa post-T. muris infection. We show that the ability to mediate expulsion resides within a subpopulation of CD4+ T cells marked by down-regulation of CD62L. These cells can be isolated from intestine-draining mesenteric lymph nodes (MLN) from day 14 post-infection, but are rare or absent in MLN before this and in spleen at all times post-infection. Among CD4+ CD62Llow MLN cells, the two most abundantly expressed chemokine receptors were CCR6 and CXCR3. We demonstrate for the first time that CD4+ CD62Llow T-cell migration to the large intestinal mucosa is dependent on the family of G alpha(i)-coupled receptors, to which chemokine receptors belong. CCR6 and CXCR3 were however dispensable for this process because neutralization of CCR6 and CXCR3 did not prevent CD4+ CD62Llow cell migration to the large intestinal mucosa during T. muris infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | T-CELLS; PROTECTIVE IMMUNITY; DENDRITIC CELLS; MICE; CD4(+); LYMPHOCYTES; EXPRESSION; RESISTANCE; PROTEINS; TISSUES; CD4; helper T cells (Th cells; Th0; Th1; Th2; Th3; Th17); chemokine receptors; helminths; mucosal immunity; trafficking |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Aug 2020 05:41 |
| Last Modified: | 10 Aug 2020 05:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25217 |
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