Ingersoll, Molly A. and Spanbroek, Rainer and Lottaz, Claudio and Gautier, Emmanuel L. and Frankenberger, Marion and Hoffmann, Reinhard and Lang, Roland and Haniffa, Muzlifah and Collin, Matthew and Tacke, Frank and Habenicht, Andreas J. R. and Ziegler-Heitbrock, Loems and Randolph, Gwendalyn J. (2010) Comparison of gene expression profiles between human and mouse monocyte subsets. BLOOD, 115 (3). E10-E19. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
Blood of both humans and mice contains 2 main monocyte subsets. Here, we investigated the extent of their similarity using a microarray approach. Approximately 270 genes in humans and 550 genes in mice were differentially expressed between subsets by 2-fold or more. More than 130 of these gene expression differences were conserved between mouse and human monocyte subsets. We con-firmed numerous of these differences at the cell surface protein level. Despite overall conservation, some molecules were conversely expressed between the 2 species' subsets, including CD36, CD9, and TREM-1. Other differences included a prominent peroxisome proliferator-activated receptor gamma (PPAR gamma) signature in mouse monocytes, which is absent in humans, and strikingly opposed patterns of receptors involved in uptake of apoptotic cells and other phagocytic cargo between human and mouse monocyte subsets. Thus, whereas human and mouse monocyte subsets are far more broadly conserved than currently recognized, important differences between the species deserve consideration when models of human disease are studied in mice. (Blood. 2010; 115: e10-e19)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN PERIPHERAL-BLOOD; DENDRITIC CELLS; SCAVENGER RECEPTOR; APOPTOTIC CELLS; MARGINAL ZONE; LYMPH-NODES; T-CELLS; MICE; IDENTIFICATION; MACROPHAGES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Aug 2020 08:23 |
| Last Modified: | 10 Aug 2020 08:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25292 |
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