Robelek, Rudolf and Wegener, J. (2010) Label-free and time-resolved measurements of cell volume changes by surface plasmon resonance (SPR) spectroscopy. BIOSENSORS & BIOELECTRONICS, 25 (5). pp. 1221-1224. ISSN 0956-5663,
Full text not available from this repository. (Request a copy)Abstract
Cell volume and its regulation is one of the key players for cellular integrity and a strong indicator for several cell pathologies. But time-resolved volume measurements of adherently grown mammalian cells using established methods, such as extracellular impedance analysis or light microscopy, are complex and time-consuming. In this study,we demonstrate that surface plasmon resonance spectroscopy (SPR) is a powerful transducer device capable of reporting volume changes of cells that are directly grown on the SPR sensor surface. The approach is label-free, non-invasive and provides an outstanding time resolution. In proof-of-principle studies we recorded the volume change of confluent MDCK 11 cells induced by hypo-or hypertonic stimulation in a time-resolved manner. Comparison of the SPR-based experiments reported here with more recent studies using different approaches suggests a direct correlation between SPR signal shift and cell volume changes. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | WATER PERMEABILITY; MICROSCOPY; CULTURE; SENSOR; ION; Surface plasmon resonance; Biosensor; Cell volume change; Label-free detection; Real-time monitoring |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Aug 2020 13:34 |
| Last Modified: | 10 Aug 2020 13:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25299 |
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