Gosau, Martin and Prantl, Lukas and Feldmann, Martina and Kokott, Andreas and Hahnel, Sebastian and Buergers, Ralf (2010) The effects of copper additives on the quantity and cell viability of adherent Staphylococcus epidermidis in silicone implants. BIOFOULING, 26 (3). pp. 359-365. ISSN 0892-7014, 1029-2454
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This in vitro study evaluated the antibacterial effect of copper additives in silicone implants. Specimens of a standard silicone material used in breast augmentation and modified copper-loaded silicone specimens were prepared and incubated in a Staphylococcus epidermidis suspension (2 h, 37 degrees C). After the quantification of adhering staphylococci using a biofluorescence assay (Resazurin), the viability of the adhering bacterial cells was quantified by live or dead cell labeling in combination with fluorescence microscopy. In the Resazurin fluorometric quantification, a higher amount of adhering S. epidermidis cells was detected on pure silicone (4612 [2319/7540] relative fluorescence units [rfu]) than on silicone with copper additives (2701 [2158/4153] rfu). Additionally, a significantly higher amount of adhering bacterial cells (5.07% [2.03%/8.93%]) was found for pure silicone than for silicone with copper additives (1.72% [1.26%/2.32%]); (p < 0.001). Calculations from live or dead staining showed that the percentage of dead S. epidermidis cells adhered on pure silicone (52.1%) was significantly lower than on silicone with copper additives (79.7%); (p < 0.001). In vitro, silicone material with copper additives showed antibacterial effects against S. epidermidis. Copper-loaded silicone may prevent bacterial colonization, resulting in lower infection rates of silicone implants.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATOMIC-FORCE MICROSCOPY; CAPSULAR CONTRACTURE; BREAST IMPLANT; AUGMENTATION RHINOPLASTY; STREPTOCOCCUS-SANGUIS; SUBCLINICAL INFECTION; SURFACE MODIFICATION; ANTIBACTERIAL; COLONIZATION; ADHESION; silicone implants; copper additives; Staphyolcoccus epidermidis; implant infection; capsular contracture; antibacterial effect |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Zahnärztliche Prothetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Aug 2020 07:22 |
| Last Modified: | 12 Aug 2020 07:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25371 |
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