Kopp, Andrea and Bala, Margarita and Weigert, Johanna and Buechler, Christa and Neumeier, Markus and Aslanidis, Charalampos and Schoelmerich, Juergen and Schaeffler, Andreas (2010) Effects of the new adiponectin paralogous protein CTRP-3 and of LPS on cytokine release from monocytes of patients with type 2 diabetes mellitus. CYTOKINE, 49 (1). pp. 51-57. ISSN 1043-4666, 1096-0023
Full text not available from this repository. (Request a copy)Abstract
Aims/Hypothesis: It was the aim to investigate the hypothesis that the new C1q/TNF-family member CTRP-3 (C1q/TNF-related protein-3) acts anti-inflammatory in human monocytes from healthy controls and patients with type 2 diabetes mellitus (T2D). Methods: Monocytes were isolated from 20 healthy controls and 30 patients with T2D. IL-6 and TNF concentrations were measured by ELISA. CTRP-3 was expressed in insect cells and used for stimulation experiments. Results: Basal IL-6 and TNF were not different in control and in T2D monocytes. LPS-stimulation (1 mu g/ml) significantly (p < 0.001) increased IL-6 and TNF in the supernatants of control and in T2D monocytes to a similar extent. CTRP-3 (1 mu g/ml) significantly (p = 0.03) inhibited LPS-induced IL-6 in control monocytes but not in T2D monocytes. TNF upon co-stimulation with LPS and CTRP-3 was significantly (p = 0.012) lower in control than in T2D monocytes. LPS-induced TNF concentration was significantly and positively correlated with serum total cholesterol and LDL cholesterol in T2D patients. Conclusions: CTRP-3 inhibits LPS-induced IL-6 and TNF release. This anti-inflammatory effect is lost in T2D. Serum cholesterol concentration affects the pro-inflammatory potential of LPS to induce TNF release from T2D monocytes in the presence or absence of CTRP-3. CTRP-3 might partly account for the pro-inflammatory state in T2D. (C) 2009 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; REPEAT-CONTAINING SEQUENCE; HIGH-DENSITY-LIPOPROTEIN; COLLAGENOUS REPEAT; 26-KDA PROTEIN; FACTOR-ALPHA; CHROMOSOMAL LOCALIZATION; PROMOTES PROLIFERATION; GENOMIC ORGANIZATION; ADIPOSE-TISSUE; Monocyte; CTRP-3; Type 2 diabetes mellitus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2020 06:12 |
| Last Modified: | 17 Aug 2020 06:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25422 |
Actions (login required)
 |
View Item |
