Tarantola, Marco and Marel, Anna-Kristina and Sunnick, Eva and Adam, Holger and Wegener, Joachim and Janshoff, Andreas (2010) Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis. INTEGRATIVE BIOLOGY, 2 (2-3). pp. 139-150. ISSN 1757-9694,
Full text not available from this repository. (Request a copy)Abstract
Early determination of the metastatic potential of cancer cells is a crucial step for successful oncological treatment. Besides the remarkable progress in molecular genomics- or proteomics-based diagnostics, there is a great demand for in vitro biosensor devices that allow rapid and selective detection of the invasive properties of tumor cells. Here, the classical cancer cell motility in vitro assays for migration and invasion relying on Boyden chambers are compared to a real-time biosensor that analyzes the dynamic properties of adherent cells electro-acoustically with a time resolution on the order of seconds. The sensor relies on the well-established quartz crystal microbalance technique (QCM) that measures the shift in resonance frequency and damping of an oscillating quartz crystal when adsorption, desorption or changes in material properties close to the quartz surface occur. In addition, the QCM is capable of detecting the rather subtle fluctuations of the cell bodies as an indicator for their micromotility. QCM-based micromotility readings of three different cancer cell lines (HT-29, HSC-4, FaDu) are compared with the well-known electrical cell-substrate impedance sensing (ECIS) revealing collective stochastic motion that corresponds to the malignancy of the cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QUARTZ-CRYSTAL MICROBALANCE; SHEAR MODE RESONATORS; SUBSTRATE INTERACTIONS; IMPEDANCE ANALYSIS; TUMOR-CELLS; IN-VITRO; MIGRATION; ADHESION; CARCINOMA; INVASION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2020 08:58 |
| Last Modified: | 17 Aug 2020 08:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25481 |
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