Janshoff, Andreas and Kunze, Angelika and Michaelis, Stefanie and Heitmann, Vanessa and Reiss, Bjoern and Wegener, Joachim (2010) Cell Adhesion Monitoring Using Substrate-Integrated Sensors. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 24 (13-14). pp. 2079-2104. ISSN 0169-4243, 1568-5616
Full text not available from this repository. (Request a copy)Abstract
Adhesion of mammalian cells to in vitro surfaces is an area of active research and it attracts considerable interest from various scientific disciplines, most notably from medical technology and biotechnology. One important issue in the context of cell-surface adhesion is the time course of attachment and spreading upon surfaces that are decorated with proteins to make them cytocompatible. This article reviews two emerging non-microscopic techniques capable of monitoring the adhesion process label-free and in real-time. Both approaches, electric cell-substrate impedance sensing (ECIS) and the quartz crystal microbalance (QCM), are based on substrate-integrated transducers that transduce cellular adhesion into an electrical signal. A short introduction of both techniques is followed by a set of examples that illustrate the performance of these sensors, their individual merits and limitations. In order to analyze the integral and complex signals of both sensors in contact with mammalian cells in more detail, we also studied their individual readouts during the adsorption of liposomes with well-defined structure and chemical composition. (C) Koninklijke Brill NV, Leiden, 2010
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QUARTZ-CRYSTAL MICROBALANCE; ELECTROCHEMICAL IMPEDANCE MEASUREMENTS; SHEAR-WAVE RESONATORS; MAMMALIAN-CELLS; ADSORPTION; ATTACHMENT; FREQUENCY; KINETICS; RESISTANCE; MONOLAYERS; Electric cell-substrate impedance sensing (ECIS); quartz crystal microbalance (QCM); cell adhesion; cell spreading; cell-matrix interactions; cell-surface junction; liposome adsorption |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Aug 2020 09:32 |
| Last Modified: | 17 Aug 2020 09:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25493 |
Actions (login required)
 |
View Item |
