Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota

Berg, Ivan A. and Ramos-Vera, W. Hugo and Petri, Anna and Huber, Harald and Fuchs, Georg (2010) Study of the distribution of autotrophic CO2 fixation cycles in Crenarchaeota. MICROBIOLOGY-SGM, 156. pp. 256-269. ISSN 1350-0872,

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Abstract

Two new autotrophic carbon fixation cycles have been recently described in Crenarchaeota. The 3-hydroxypropionate/4-hydroxybutyrate cycle using acetyl-coenzyme A (CoA)/propionyl-CoA carboxylase as the carboxylating enzyme has been identified for (micro)aerobic members of the Sulfolobales. The dicarboxylate/4-hydroxybutyrate cycle using oxygen-sensitive pyruvate synthase and phosphoenolpyruvate carboxylase as carboxylating enzymes has been found in members of the anaerobic Desulfurococcales and Thermoproteales. However, Sulfolobales include anaerobic and Desulfurococcales aerobic autotrophic representatives, raising the question of which of the two cycles they use. We studied the mechanisms of autotrophic CO2 fixation in the strictly anaerobic Stygiolobus azoricus (Sulfolobales) and in the facultatively aerobic Pyrolobus fumarii (Desulfurococcales). The activities of all enzymes of the 3-hydroxypropionate/4-hydroxybutyrate cycle were found in the anaerobic S. azoricus. In contrast, the aerobic or denitrifying P. fumarii possesses all enzyme activities of the dicarboxylate/4-hydroxybutyrate cycle. We conclude that autotrophic Crenarchaeota use one of the two cycles, and that their distribution correlates with the 16S rRNA-based phylogeny of this group, rather than with the aerobic or anaerobic lifestyle.

Item Type: Article
Uncontrolled Keywords: TRICARBOXYLIC-ACID CYCLE; COMPLETE GENOME SEQUENCE; CARBON-DIOXIDE ASSIMILATION; RUBISCO-LIKE PROTEINS; SP-NOV REPRESENTS; HYDROGENOBACTER-THERMOPHILUS; 3-HYDROXYPROPIONATE CYCLE; METALLOSPHAERA-SEDULA; IGNICOCCUS-HOSPITALIS; THERMOACIDOPHILIC ARCHAEBACTERIA;
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 24 Aug 2020 05:09
Last Modified: 24 Aug 2020 05:09
URI: https://pred.uni-regensburg.de/id/eprint/25543

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