Schmidt, Florian and Schmidt, Jennifer and Riechers, Alexander and Haase, Susann and Bosserhoff, Anja-Katrin and Heilmann, Joerg and Koenig, Burkhard (2010) DNA Staining in Agarose Gels with ZN2+-Cyclen-Pyrene. NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 29 (10): PII 927597. pp. 748-759. ISSN 1525-7770,
Full text not available from this repository. (Request a copy)Abstract
A pyrene-labeled Zn2+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amounts as low as 10 ng and short DNA strands (300 b.p.) are detectable. Gel extraction as a standard technique in molecular biology was successfully performed after staining with Zn2+-cyclen-pyrene. Cytotoxicity tests on HeLa and V-79 cells reveal that the zinc-cyclen pyrene probe is significant less toxic compared to ethidium bromide.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGHLY SELECTIVE RECOGNITION; ETHIDIUM-BROMIDE; PROTEIN-PHOSPHORYLATION; FRAMESHIFT MUTATIONS; AQUEOUS-SOLUTION; NUCLEIC ACIDS; SDS-PAGE; COMPLEX; DECONTAMINATION; THYMIDINE; DNA staining; Zn2+-cyclen; pyrene; excimer emission |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Pathologie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Aug 2020 06:41 |
| Last Modified: | 24 Aug 2020 06:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25570 |
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