Schaumburger, J. and Beckmann, J. and Springorum, H. -R. and Handel, M. and Anders, S. and Kalteis, T. and Grifka, J. and Rath, B. (2010) Toxicity of Antiseptics on Chondrocytes in vitro. ZEITSCHRIFT FUR ORTHOPADIE UND UNFALLCHIRURGIE, 148 (1). pp. 39-43. ISSN 1864-6697,
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Aim: Local antiseptics are commonly used for perioperative skin and wound disinfection and as solutions for joint lavage. Therefore, we examined if an intra-articular use of these antiseptics is possible by using an in vitro chondrocyte model. Material and Methods: Articular chondrocytes harvested from 7 patients were cultured. After reaching 80% confluency different concentrations (0%, 1%, 10%, 50%, 100%) of polyhexanide, hydrogen peroxide and povidone-iodine were added for 5 minutes. Afterwards, the solution was removed and the chondrocytes were cultured for 24 hours. Subsequently the vitality and proliferation rate (DNA synthesis) were analysed with the WST-1 and BrdU tests. Results: 1% povidone-iodine and 1% hydrogen peroxide solutions significantly (p = 0.001) decreased the chondrocyte vitality as compared to our control group. There was no significant difference (p = 0.71) after the application of 1% polyhexanide in the vitality ratios. A significant decrease in vitality was also observed after the application of 10% polyhexanide solution (p = 0.001). Application of 1% povidone-iodine solution, 1% hydrogen peroxide solution and 10% polyhexanide revealed a decrease in the metabolic cell activity of 80% compared to our control group, whereas the activity was 65% (p = 0.026) compared to the control group after application of 1% polyhexanide solution. Conclusion: Our results demonstrate the chondrotoxic effect of the tested antiseptic solutions in clinical used concentrations within short time points. Polyhexanide in a low concentrated solution (1%) was the antiseptic with the lowest influence on the vitality and the DNA synthesis of chondrocytes. Thus, this antiseptical solution seemed to be the best choice for intra-articular application. But overall, our study showed general limitations for the intra-articular use of local antiseptics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OSTEOBLASTS; THERAPY; CELLS; SKIN; local antiseptic; tissue compatibility; chondrocytes; polyhexanide; povidone-iodine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Aug 2020 08:17 |
| Last Modified: | 27 Aug 2020 08:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25640 |
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